CONSERVATION OF PLANT MATERIAL IN ULTRA LOW TEMPERATURES

Cryopreservation

Long-term storage of genetic material

Cryopreservation is a method that allows in situ long-term storage of genetic material with the use of minimum space. The ultra-low temperature of liquid nitrogen (-196 °C) guarantees conservation of plant material, using storage tanks of freezers as gene banks.

Key Features

  • Safe storage of plants
  • Minimal use of space
  • Protocol development
  • Cryopreservation training

Key Features

  • Safe storage of plants
  • Minimal use of space
  • Protocol development
  • Cryopreservation training

Storage of plant material is important to backup or secure the future availability of high quality genetics, and medicinally important cultivars with unique attributes. This is useful for breeding programs, pharmaceuticals, and research. The major advantage of this procedure is to diminish in vitro culture of manual propagation costs, required space and maintenance, contamination, and somaclonal variation risk. Shoot tips (or apical meristems) of tissue culture-grown plantlets are treated to determine an ice-free intracellular environment, necessary to maintain plants in stable metabolic condition during long-term storage in liquid nitrogen.

What we offer

ViVi can help you develop a working protocol, with detailed step-by-step instructions to perform the entire cryopreservation procedure. Our researcher will assist you through both on-site and remote training in to how to use the equipment, and how to perform every step: starting from preparation of the explants (meristem excision), to pre-treatment, freezing, thawing, and revival of the plant material. We will provide recommendations for all the equipment, tools, and chemicals needed. Please contact us for more information.

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